RNA
Part:BBa_K1555007:Design
Designed by: Xu Xuan Group: iGEM14_NJAU_China (2014-10-15)
copA's 20nt target sequence+sgRNA scaffold
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used overlap PCR to link 20nt target sequence with sgRNA scaffold.
Source
20nt target sequence was synthesized artificially
the scaffold was acquired by PCR from a plasimid made by CAS